New publication in the journal Blood
New publication by Felix Ellett, Luke Pase, John Hayman and Graham Lieschke published in the journal Blood. Felix, Luke, John and Graham recently moved from the WEHI to ARMI.
mpeg1 promoter transgenes direct macrophage-lineage expression in
zebrafish
Felix Ellett1,
Luke Pase1,
John W. Hayman1,
Alex Andrianopoulos2 and
Graham J. Lieschke1,*
1Cancer and Haematology Division, The Walter and Eliza Hall
Institute of Medical Research, Melbourne, Victoria, Australia;
2Department of Genetics, University of Melbourne,
Melbourne, Victoria, Australia
Abstract
Macrophages and neutrophils play important roles during the innate
immune response, phagocytosing invading microbes and delivering
antimicrobial compounds to the site of injury. Functional analyses
of the cellular innate immune response in zebrafish infection/inflammation
models have been aided by transgenic lines with
fluorophore-marked neutrophils. However, it has not been
possible to study macrophage behaviors and neutrophil/macrophage interactions
in vivo directly because there has been no macrophage-only reporter
line.
To remove this roadblock, a macrophage-specific marker
was identified (mpeg1) and its promoter used in mpeg1-driven
transgenes. mpeg1-driven transgenes are expressed in
macrophage-lineage cells that do not express
neutrophil-marking transgenes. Using these lines, the
different dynamic behaviors of neutrophils and macrophages
following wounding were compared side-by-side in compound
transgenics. Macrophage/neutrophil interactions, such as
phagocytosis of senescent neutrophils were readily observed in
real time. These zebrafish transgenes provide a new resource that
will contribute to the fields of inflammation, infection and
leukocyte biology.
Source: http://bloodjournal.hematologylibrary.org/cgi/content/abstract/blood-2010-10-314120v1
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