Human pluripotent stem cells (hPSCs) have the ability of indefinite self-renewal and to differentiate into all types of human adult cells.

The Laslett group investigates the biology of human pluripotent stem cell lines, including embryonic stem cells (hESC) and human induced pluripotent stem cells (iPS) – developed cells that have been manipulated to enter a more primitive, less specialised stem cell state.

Research

More understanding of human pluripotent stem cell lines will lead to the development of tools and novel cell lines that will be required for the safe use of these cell types in future cell-based industries.

This is important because although the potential for the treatment of diseases and injuries is huge, the technology also creates a number of risks when producing cell populations to be used for cell therapy.

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  • The production and characterisation of monoclonal antibodies that detect live human pluripotent stem cells
  • Functional uses for monoclonal antibodies that detect live human pluripotent stem cells
  • The development of “disease in a dish” models using human induced pluripotent stem cells
  • Investigation of culture substrates for the maintenance and differentiation of human pluripotent stem cells.

Featured Publications

More Publications

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Murphy AR, Laslett AL, O’Brien CM and Cameron, NR.

Scaffolds for 3D In Vitro Culture of Neural Lineage Cells.

Acta Biomaterialia (in press March 2017).

Wang YC, Stein JW, Lynch CL, Tran HT, Lee CY, Coleman R, Hatch A, Antontsev V, Chy HS, O’Brien C, Murthy SK, Laslett AL, Peterson SE, Loring JF .

Glycosyltransferase ST6GAL1 contributes to the regulation of pluripotency in human pluripotent stem cells.

Sci Rep. 2015 Aug 25;5:13317. doi: 10.1038/srep13317.

Mason EA, Mar JC, Laslett AL, Pera MF, Quackenbush J, Wolvetang E, Wells CA.

Gene expression variability as a unifying element of the pluripotency network.

Stem Cell Reports. 2014 Aug 12;3(2):365-77. doi: 10.1016/j.stemcr.2014.06.008. Epub 2014 Jul 25.

Tian W, Wang Y, Xu Y, Guo X, Wang B, Sun L, Liu L, Cui F, Zhuang Q, Bao X, Schley G, Chung TL, Laslett AL, Willam C, Qin B, Maxwell PH, Esteban MA.

The hypoxia-inducible factor renders cancer cells more sensitive to vitamin C-induced toxicity.

J Biol Chem. 2014 Feb 7;289(6):3339-51. doi: 10.1074/jbc.M113.538157. Epub 2013 Dec 26.

Tian W, Wang Y, Xu Y, Guo X, Wang B, Sun L, Liu L, Cui F, Zhuang Q, Bao X, et al.

Hypoxia-Inducible Factor Renders Cancer Cells More Sensitive to Vitamin C-Induced Toxicity.

J Biol Chem. 2014 Feb 7;289(6):3339-51. doi: 10.1074/jbc.M113.538157. Epub 2013 Dec 26.

Polanco JC, Wang B, Zhou Q, Chy H, O'Brien C, Laslett AL.

Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2.

J Vis Exp. 2013 Dec 6;(82):50856. doi: 10.3791/50856. Epub 2013 Dec 6.

Xu Y, Liu L, Laslett AL, Esteban MA.

Cell Reprogramming: Into the groove.

Nat Mater. 2013 Dec;12(12):1082-4. doi: 10.1038/nmat3821.

Polanco JC, and Laslett AL.

Safety assessment of reprogrammed cells prior to clinical applications: Potential approaches to eliminate teratoma formation.

Pluripotent Stem Cells, 2015 Sep 30;25:555-566. Doi: 10.5772/54400

Polanco JC, Ho MSH, Wang B, Zhou Q, Wolvetang E, Mason E, Wells CA, Kolle G, Grimmond SM, Bertoncello I, et al.

Identification of Unsafe Human Induced Pluripotent Stem Cell Lines Using a Robust Surrogate Assay for Pluripotency.

Stem Cells. 2013 Aug;31(8):1498-510. doi: 10.1002/stem.1425.

Lambshead JW, Meagher L, O’Brien C, and Laslett AL.

Defining synthetic surfaces for human pluripotent stem cell culture.

Cell Regen 2013;2(1):7. doi 10.1186/2045-9769-2-7. Epub 2013 Nov 22.

O’Brien C, Lambshead J, Chy H, Zhou Q, Wang Y-C, and Laslett AL.

Analysis and purification techniques for human pluripotent stem cells.

Human Stem Cell Manual: A Laboratory Guide, Volume 2nd Edition, J.F.L.S.E. Petersen, ed. (Elsevier), pp. p223-248.

Nazor KL, Altun G, Lynch C, Tran H, Harness JV, Slavin I, Garitaonandia I, Müller FJ, Wang YC, Boscolo FS, Fakunle E, Dumevska B, Lee S, Park HS, Olee T, D'Lima DD, Semechkin R, Parast MM, Galat V, Laslett AL, Schmidt U, Keirstead HS, Loring JF, Laurent LC.

Recurrent Variations in DNA Methylation in Human Pluripotent Stem Cells and Their Differentiated Derivatives.

Cell Stem Cell. 2012 May 4;10(5):620-34. doi: 10.1016/j.stem.2012.02.013.

Laurent LC, Ulitsky I, Slavin I, Tran H, Schork A, Morey R, Lynch C, Harness JV, Lee S, Barrero MJ, Ku S, Martynova M, Semechkin R, Galat V, Gottesfeld J, Izpisua Belmonte JC, Murry C, Keirstead HS, Park HS, Schmidt U, Laslett AL, Muller FJ, Nievergelt CM, Shamir R, Loring JF.

Dynamic Changes in the Copy Number of Pluripotency and Cell Proliferation Genes in Human ESCs and iPSCs during Reprogramming and Time in Culture.

Cell Stem Cell. 2011 Jan 7;8(1):106-18. doi: 10.1016/j.stem.2010.12.003.

Kolle G, Shepherd JL, Gardiner B, Kassahn KS, Cloonan N, Wood DLA, Nourbakhsh E, Taylor DF, Wani S, Chy HS, et al.

Deep-transcriptome and ribonome sequencing redefines the molecular networks of pluripotency and the extracellular space in human embryonic stem cells.

Genome Res 2011 August 23;21:2014-2025. doi: 10.1101/gr.119321.110.

Amps K, Andrews PW, Anyfantis G, Armstrong L, Avery S, Baharvand H, Baker J, Baker D, Munoz MB, Beil S, et al.

Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage.

Nat Biotechnol. 2011 Nov 27;29(12):1132-44. doi: 10.1038/nbt.2051.

Laurent LC, Nievergelt CM, Lynch C, Fakunle E, Harness JV, Schmidt U, Galat V, Laslett AL, Otonkoski T, Keirstead HS, Schork A, Park HS, Loring JF.

Restricted ethnic diversity in human embryonic stem cell lines.

Nat Methods. 2010 Jan;7(1):6-7. doi: 10.1038/nmeth0110-06.

Herszfeld D, Wolvetang E, Langton-Bunker E, Chung TL, Filipczyk AA, Houssami S, Jamshidi P, Koh K, Laslett AL, Michalska A, Nguyen L, Reubinoff BE, Tellis I, Auerbach JM, Ording CJ, Looijenga LH, Pera MF.

CD30 is a survival factor and a biomarker for transformed human pluripotent stem cells.

Nat Biotechnol. 2006 Mar;24(3):351-7. Epub 2006 Feb 26.