Nadinath Nillegoda



Research interests

For more information, visit my PubMed and Google Scholar.

Featured Publications

Authors Title Published In

van Oosten-Hawle P, Backe SJ, Ben-Zvi A, Bourboulia D, Brancaccio M, Brodsky J, Clark M, Colombo G, Cox MB, De Los Rios P, Echtenkamp F, Edkins A, Freeman B, Goloubinoff P, Houry W, Johnson J, LaPointe P, Li W, Mezger V, Neckers L, Nillegoda NB, Prahlad V, Reitzel A, Scherz-Shouval R, Sistonen L, Tsai FTF, Woodford MR, Mollapour M, Truman AW.

Second Virtual International Symposium on Cellular and Organismal Stress Responses, September 8-9, 2022.

Cell Stress Chaperones. 2023 Jan 5. doi: 10.1007/s12192-022-01318-5. Online ahead of print.

Zhang R, Malinverni D, Cyr DM, Rios PL, Nillegoda NB.

J-domain protein chaperone circuits in proteostasis and disease.

Trends Cell Biol. 2023 Jan;33(1):30-47. doi: 10.1016/j.tcb.2022.05.004.

Cox D, Ang CS, Nillegoda NB, Reid GE, Hatters DM.

Hidden information on protein function in censuses of proteome foldedness.

Nat Commun. 2022 Apr 14;13(1):1992. doi: 10.1038/s41467-022-29661-2.

Mathangasinghe Y, Fauvet B, Jane SM, Goloubinoff P, Nillegoda NB.

The Hsp70 chaperone system: distinct roles in erythrocyte formation and maintenance.

Haematologica. 2021 Jun 1;106(6):1519-1534. doi: 10.3324/haematol.2019.233056.

Wentink AS, Nillegoda NB, Feufel J, Ubartaitė G, Schneider CP, De Los Rios P, Hennig J, Barducci A, Bukau B.

Molecular dissection of amyloid disaggregation by human HSP70.  

Nature. 2020 Nov 11. doi: 10.1038/s41586-020-2904-6. Online ahead of print.

Serlidaki D, van Waarde MAWH, Rohland L, Wentink AS, Dekker SL, Kamphuis MJ, Boertien JM, Brunsting JF, Nillegoda NB, Bukau B, Mayer MP, Kampinga HH, Bergink S

Functional diversity between HSP70 paralogs caused by variable interactions with specific co-chaperones.

J Biol Chem. 2020 May 22;295(21):7301-7316. doi: 10.1074/jbc.RA119.012449. Epub 2020 Apr 13. PMID: 32284329

Alberts N, Mathangasinghe Y, Nillegoda NB.

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells.

J Vis Exp. 2019 Sep 2;(151). doi: 10.3791/60172.

Rosenzweig R*, Nillegoda NB*, Mayer MP* and Bukau B*.  (* Equal contribution)

The Hsp70 chaperone network.

Nat Rev Mol Cell Biol.  2019 in press.

Nillegoda NB, Wentink AS, Bukau B.

Protein disaggregation in multicellular organisms.

Trends Biochem Sci. 2018 Apr;43(4):285-300. doi: 10.1016/j.tibs.2018.02.003. Epub 2018 Feb 28.

Kirstein J, Arnsburg K, Scior A, Szlachcic A, Guilbride DL, Morimoto RI, Bukau B, Nillegoda NB.

In vivo properties of the disaggregase function of J-proteins and Hsc70 in Caenorhabditis elegans stress and aging.

Aging Cell. 2017 Dec;16(6):1414-1424. doi: 10.1111/acel.12686. Epub 2017 Oct 10.

Garcia VM, Nillegoda NB, Bukau B, Morano KA.

Substrate binding by the yeast Hsp110 nucleotide exchange factor and molecular chaperone Sse1 is not obligate for its biological activities.

Mol Biol Cell. 2017 Jul 15;28(15):2066-2075. doi: 10.1091/mbc.E17-01-0070. Epub 2017 May 24.

Nillegoda NB, Stank A, Malinverni D, Alberts N, Szlachcic A, Barducci A, De Los Rios P, Wade RC, Bukau B.

Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes.

Elife. 2017 May 15;6. pii: e24560. doi: 10.7554/eLife.24560.

Żwirowski S, Kłosowska A, Obuchowski I, Nillegoda NB, Piróg A, Ziętkiewicz S, Bukau B, Mogk A, Liberek K.

Hsp70 displaces small heat shock proteins from aggregates to initiate protein refolding.

EMBO J. 2017 Mar 15;36(6):783-796. doi: 10.15252/embj.201593378. Epub 2017 Feb 20.

Nillegoda NB, Kirstein J, Szlachcic A, Berynskyy M, Stank A, Stengel F, Arnsburg K, Gao X, Scior A, Aebersold R, Guilbride DL, Wade RC, Morimoto RI, Mayer MP, Bukau B.

Crucial HSP70 co-chaperone complex unlocks metazoan protein disaggregation.

Nature. 2015 Aug 13;524(7564):247-51. doi: 10.1038/nature14884. Epub 2015 Aug 5.

Gao X, Carroni M, Nussbaum-Krammer C, Mogk A, Nillegoda NB, Szlachcic A, Guilbride DL, Saibil HR, Mayer MP, Bukau B.

Human Hsp70 disaggregase reverses Parkinson’s-linked α-Synuclein amyloid fibrils.

Mol Cell. 2015 Sep 3;59(5):781-93. doi: 10.1016/j.molcel.2015.07.012. Epub 2015 Aug 20.

Nillegoda NB, Bukau B.

Metazoan Hsp70-based protein disaggregases: emergence and mechanisms.

Front Mol Biosci. 2015 Oct 9;2:57. doi: 10.3389/fmolb.2015.00057. eCollection 2015.

Hsieh TY, Nillegoda NB, Tyedmers J, Bukau B, Mogk A, Kramer G.

Monitoring protein misfolding by site-specific labeling of proteins in vivo.

PLoS One. 2014 Jun 10;9(6):e99395. doi: 10.1371/journal.pone.0099395. eCollection 2014.

Theodoraki MA, Nillegoda NB, Saini J, Caplan AJ.

A network of ubiquitin ligases is important for the dynamics of misfolded protein aggregates in yeast.

J Biol Chem. 2012 Jul 6;287(28):23911-22. doi: 10.1074/jbc.M112.341164. Epub 2012 May 16.

Rampelt H, Kirstein-Miles J, Nillegoda NB, Chi K, Scholz SR, Morimoto R, Bukaua B.

Metazoan Hsp70 machines use Hsp110 to power protein disaggregation.

EMBO J. 2012;31(21):4221-4235. doi:10.1038/emboj.2012.264.

Mandal AK, Theodoraki MA, Nillegoda NB, Caplan AJ.

Role of molecular chaperones in biogenesis of the protein kinome.

Methods Mol Biol. 2011;787:75-81. doi: 10.1007/978-1-61779-295-3_6.

Nillegoda NB, Theodoraki MA, Mandal AK, Mayo KJ, Ren HY, Sultana R, Wu K, Johnson J, Cyr DM, Caplan AJ.

Ubr1 and Ubr2 function in a quality control pathway for degradation of unfolded cytosolic proteins.

Mol Biol Cell. 2010 Jul 1;21(13):2102-16. doi: 10.1091/mbc.E10-02-0098. Epub 2010 May 12.

Mandal AK, Gibney PA, Nillegoda NB, Theodoraki MA, Caplan AJ, Morano KA.

Hsp110 chaperones control client fate determination in the hsp70-Hsp90 chaperone system.

Mol Biol Cell. 2010 May 1;21(9):1439-48. doi: 10.1091/mbc.E09-09-0779. Epub 2010 Mar 17.

Mandal AK, Nillegoda NB, Chen JA, Caplan AJ.

Ydj1 protects nascent protein kinases from degradation and controls the rate of their maturation.

Mol Cell Biol. 2008 Jul;28(13):4434-44. doi: 10.1128/MCB.00543-08. Epub 2008 Apr 28.

Jones J, Wu K, Yang Y, Guerrero C, Nillegoda NB, Pan ZQ, Huang L.

A targeted proteomic analysis of the ubiquitin-like modifier nedd8 and associated proteins.

J Proteome Res. 2008 Mar;7(3):1274-87. doi: 10.1021/pr700749v. Epub 2008 Feb 5.

Mandal AK, Lee P, Chen JA, Nillegoda NB, Heller A, DiStasio S, Oen H, Victor J, Nair DM, Brodsky JL, Caplan AJ.

Cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturation.

J Cell Biol. 2007 Jan 29;176(3):319-28. Epub 2007 Jan 22.